Transgenic and targeted mouse mutation database
نویسندگان
چکیده
منابع مشابه
MouseBank: a database application for managing transgenic mouse breeding programs.
We have developed a powerful tool for managing transgenic and knockout mouse programs using the Macintoshand Microsoft Windows-compatible relational database management system, 4th Dimension (4D). MouseBank is a user-friendly system designed for small laboratories and large workgroups to organize every aspect of the generation and breeding of transgenic animals. MouseBank is built on the cl...
متن کاملProliferation is necessary for both repair and mutation in transgenic mouse cells.
Proliferating cells are often presumed to be more mutable than quiescent cells because they have less time to repair DNA damage before DNA replication. Direct tests of this hypothesis have been confounded by the need for cell division before a mutation can be detected. We have avoided this problem by showing that the Big Blue mouse cell line permits the dynamic quantification of both lesions an...
متن کاملTargeted mutation of mouse skeletal muscle sodium channel produces myotonia and potassium-sensitive weakness.
Hyperkalemic periodic paralysis (HyperKPP) produces myotonia and attacks of muscle weakness triggered by rest after exercise or by K+ ingestion. We introduced a missense substitution corresponding to a human familial HyperKPP mutation (Met1592Val) into the mouse gene encoding the skeletal muscle voltage-gated Na+ channel NaV1.4. Mice heterozygous for this mutation exhibited prominent myotonia a...
متن کاملTargeted Mutation of the Mouse Grp94 Gene Disrupts Development and Perturbs Endoplasmic Reticulum Stress Signaling
Glucose-regulated protein 94 (GRP94) is one of the most abundant endoplasmic reticulum (ER) resident proteins and is the ER counterpart of the cytoplasmic heat shock protein 90 (HSP90). GRP94, a component of the GRP78 chaperone system in protein processing, has pro-survival properties with implicated function in cancer progression and autoimmune disease. Previous studies on the loss of GRP94 fu...
متن کاملc-Myc targeted regulators of cell metabolism in a transgenic mouse model of papillary lung adenocarcinoma
c-Myc's role in pulmonary cancer metabolism is uncertain. We therefore investigated c-Myc activity in papillary lung adenocarcinomas (PLAC). Genomics revealed 90 significantly regulated genes (> 3-fold) coding for cell growth, DNA metabolism, RNA processing and ribosomal biogenesis and bioinformatics defined c-Myc binding sites (TFBS) at > 95% of up-regulated genes. EMSA assays at 33 novel TFBS...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Genome Biology
سال: 2001
ISSN: 1474-760X
DOI: 10.1186/gb-2001-2-8-reports2005